jerry submitted, created time 4 months 2 weeks (www.developmentalcell.com)

Serving as the primary conduit for communication between the nucleus and the cytoplasm, nuclear pore complexes (NPCs) impact nearly every cellular process. The extent to which NPC composition varies and the functional significance of such variation in mammalian development has not been investigated. Here they report that a null allele of mouse nucleoporin Nup133, a structural subunit of the NPC, disrupts neural differentiation. They found that expression of Nup133 was cell type and developmental stage restricted, with prominent expression in dividing progenitors. Nup133-deficient epiblast and ES cells abnormally maintain features of pluripotency and differentiate inefficiently along the neural lineage. Neural progenitors achieve correct spatial patterning in mutant embryos; however, they are impaired in generating terminally differentiated neurons, as are Nup133 null ES cells. This results reveal a role for structural nucleoporins in coordinating cell differentiation events in the developing embryo.

Keywords: nuclear pore complexes nucleoporin stem cells embryos neural stem cells