Translation factors promote the formation of two states of the closed-loop mRNP

kavin submitted, created time 5 months 3 weeks (www.ncbi.nlm.nih.gov)

In the paper, the authors use toeprinting and polysome profiling assays to delineate ribosome positioning at initiator AUG codons and ribosome-mRNA association, respectively, and find that two distinct stable (resistant to cap analogue) closed-loop structures are formed during initiation in yeast cell-free extracts. The integrity of both forms requires the mRNA cap and poly(A) tail, as well as eIF4E, eIF4G, Pab1 and eIF3, and is dependent on the length of both the mRNA and the poly(A) tail.

Keywords: Pab1 eIF3 eIF4G eIF4E mRNA AUG ribosomes polysomes translation mRNP closed-loop mRNP Translation factors