Generation of DNA cleavage specificities of type II restriction endonucleases by reassortment of target recognition domains

annatto submitted, created time 1 year 7 months (www.pnas.org)

"Type II restriction endonucleases (REases) cleave double-stranded DNA at specific sites within or close to their recognition sequences. Shortly after their discovery in 1970, REases have become one of the primary tools in molecular biology. However, the list of available specificities of type II REases is relatively short despite the extensive search for them in natural sources and multiple attempts to artificially change their specificity

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Immune complexes of auto-antibodies against Abold italic beta1-42 peptides patrol cerebrospinal fluid of non-Alzheimer's patients

angelfish submitted, created time 1 year 7 months (www.nature.com)

"The diagnostic potential of large Abeta-peptide binding particles (LAPs) in the cerebrospinal fluid (CSF) of Alzheimer's dementia (AD) patients and non-AD controls (nAD) was evaluated. LAPs were detected by confocal spectroscopy in both groups with high inter-individual variation in number. Molecular imaging by confocal microscopy revealed that LAPs are heterogeneous superaggregates that could be subdivided morphologically into four main types (LAP1–4). LAP-4 type, resembling a 'large chain of pearls', was detected in 42.1% of all nAD controls but it was virtually absent in AD patients

Submit comment | Blog It | Email It | Keywords: immunoglobulin G (IgG) LAP-4 type cerebrospinal fluid