Hydrogen Bonds of RNA Are Stronger than Those of DNA, but NMR Monitors Only Presence of Methyl Substituent in Uracil/Thymine, not Bond Strength

Novel drug discovery and molecular biological methods, via DNA, RNA and protein changes using structure–function transitions: Transitional structural chemogenomics, transitional structural nucleic acid microarray

DNAMENYIT submitted, created time 1 year 4 months (iris.nyit.edu)

Nucleic acids and proteins are dynamic molecules that undergo structural changes which control gene expression. The authors have developed two novel techniques, viz., transitional structural chemogenomics and
transitional structural chemoproteomics. Transitional structural chemogenomics is used to regulate gene expression, employing ultrasensitive small-molecule drugs targeted toward nucleic acids. Gene expression can be regulated by
using chemicals to target transitional changes in the helical conformations of single-stranded (ss-) and double-stranded (ds-) DNA (e.g

4 comments | Blog It | Email It | Keywords: RNA, DNA,GENE single-stranded DNA gene expression microarrays RNA-DNA hybrid stability gene expression microarray Nuclear Gene Expression gene expression profile RNA, DNA,GENE Single-Stranded DNA gene expression microarrays RNA-DNA hybrid stability gene expression microarray Nuclear Gene Expression gene expression profile

On the stability of peptide nucleic acid duplexes in the presence of organic solvents

gh0706 submitted, created time 1 year 6 months (nar.oxfordjournals.org)

It would be predicted that the hydrogen bonding contributions to increase and the stacking contributions to decrease as the water activity in the medium decreases. Study of nucleobase paired duplexes in the absence of water and ultimately in pure aprotic, non-polar organic solvents is not possible with natural phosphodiester nucleic acids due to the ionic phosphate groups and the associated cations, but could be possible with non-ionic nucleic acid analogues or mimics such as peptide nucleic acids

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Hairpin extensions enhance the efficacy of mycolyl transferase-specific antisense oligonucleotides targeting Mycobacterium tuberculosis

diggman submitted, created time 1 year 8 months (www.pnas.org)

" e have investigated the efficacy of modifying gene-specific antisense phosphorothioate oligodeoxyribonucleotides (PS-ODNs) by the addition of 5' and 3' hairpin extensions. As a model system, we have targeted the Mycobacterium tuberculosis 30/32-kDa mycolyl transferase protein complex genes encoding three highly related enzymes (antigens 85 A, B, and C). Whereas the addition of a hairpin extension at only one end of the PS-ODNs did not improve their inhibitory capacity, the addition of hairpin extensions at both ends enhanced their capacity to inhibit M

Submit comment | Blog It | Email It | Keywords: antisense oligodeoxyribonucleotide thera RNA-DNA hybrid stability antituberculous antibiotics species-specific targets