The SNARE Complex from Yeast Is Partially Unstructured on the Membrane

jerry submitted, created time 4 months 6 days (www.structure.org)

Molecular recognition between cognate SNAREs leads to the formation of a four-helix bundle, which facilitates vesicle docking and membrane fusion. For a SNARE system involved in trafficking in yeast, target membrane (t-) SNARE Sso1p and vesicle associated (v-) SNARE Snc2p contribute one SNARE motif each, whereas another t-SNARE (Sec9) donates two N-terminal and C-terminal SNARE motifs (SN1 and SN2) to the helical bundle. By use of EPR, it is found that SN2 has a tendency to be uncoiled, leaving a significant population of the SNARE complexes to be partially unstructured on the membrane

2 comments | Blog It | Email It | Keywords: Sso1p Snc2p Sec9 membranes vesicle docking SNARE membrane fusion SN2 SNARE protein