The SNARE Complex from Yeast Is Partially Unstructured on the Membrane

jerry submitted, created time 4 months 6 days (www.structure.org)

Molecular recognition between cognate SNAREs leads to the formation of a four-helix bundle, which facilitates vesicle docking and membrane fusion. For a SNARE system involved in trafficking in yeast, target membrane (t-) SNARE Sso1p and vesicle associated (v-) SNARE Snc2p contribute one SNARE motif each, whereas another t-SNARE (Sec9) donates two N-terminal and C-terminal SNARE motifs (SN1 and SN2) to the helical bundle. By use of EPR, it is found that SN2 has a tendency to be uncoiled, leaving a significant population of the SNARE complexes to be partially unstructured on the membrane

2 comments | Blog It | Email It | Keywords: Sso1p Snc2p Sec9 membranes vesicle docking SNARE membrane fusion SN2 SNARE protein

Identification of the Exocytic Machinery Associated with the Natural Killer Cell Secretory Lysosome

annatto submitted, created time 1 year 5 months (www.mcponline.org)

"Natural killer (NK) cells and cytotoxic T lymphocytes eliminate virally infected and transformed cells. Target cell killing is mediated by the regulated exocytosis of secretory lysosomes, which deliver perforin and proapoptotic granzymes to the infected or transformed cell. Yet despite the central role that secretory lysosome exocytosis plays in the immune response to viruses and tumors, little is known about the molecular machinery that regulates the docking and fusion of this organelle with the plasma membrane

Submit comment | Blog It | Email It | Keywords: NK cells Natural killer (NK) cells SNARE LC ESI-MS&MS