Translation factors promote the formation of two states of the closed-loop mRNP

kavin submitted, created time 6 months 1 week (www.ncbi.nlm.nih.gov)

In the paper, the authors use toeprinting and polysome profiling assays to delineate ribosome positioning at initiator AUG codons and ribosome-mRNA association, respectively, and find that two distinct stable (resistant to cap analogue) closed-loop structures are formed during initiation in yeast cell-free extracts. The integrity of both forms requires the mRNA cap and poly(A) tail, as well as eIF4E, eIF4G, Pab1 and eIF3, and is dependent on the length of both the mRNA and the poly(A) tail.

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EcoTILLING for the identification of allelic variants of melon eIF4E, a factor that controls virus susceptibility

crackpot submitted, created time 1 year 5 months (www.biomedcentral.com)

"This study shows the applicability of EcoTILLING in Cucumis spp., but given the conservation of eIF4E, new candidate genes should probably be considered to identify new sources of resistance to plant viruses. Part of the methodology described here could alternatively be used in TILLING experiments that serve to generate new eIF4E alleles."

Submit comment | Blog It | Email It | Keywords: eIF4E Resistance Genes epidermal growth factor receptor (EGFR) eIF4E