Stochastic inhibitor release and binding from single-enzyme molecules

herry submitted, created time 1 year 2 months (www.pnas.org)

Inhibition kinetics of single-β-galactosidase molecules with the slow-binding inhibitor D-galactal have been characterized by segregating individual enzyme molecules in an array of 50,000 ultra small reaction containers and observing substrate turnover changes with fluorescence microscopy.

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Single-molecule measurements of the opening and closing of the DNA gate by eukaryotic topoisomerase II

collapsar submitted, created time 1 year 9 months (www.pnas.org)

Type II DNA topoisomerases are essential and ubiquitous enzymes that perform important functions in chromosome condensation and segregation and in regulating intracellular DNA supercoiling. Topoisomerases carry out these DNA transactions by passing one segment of DNA through the other by using a reversible, enzyme-bridged double strand break. The transient enzyme/DNA adduct is mediated by a phosphodiester bond between the active-site tyrosine and a backbone phosphate of DNA

Submit comment | Blog It | Email It | Keywords: fluorescence microscopy single-molecule kinetics FRET enzyme dynamics