Nanotechnology Innovation May Revolutionize Gene Detection in a Single Cell

Novel drug discovery and molecular biological methods, via DNA, RNA and protein changes using structure–function transitions: Transitional structural chemogenomics, transitional structural nucleic acid microarray

DNAMENYIT submitted, created time 1 year 4 months (iris.nyit.edu)

Nucleic acids and proteins are dynamic molecules that undergo structural changes which control gene expression. The authors have developed two novel techniques, viz., transitional structural chemogenomics and
transitional structural chemoproteomics. Transitional structural chemogenomics is used to regulate gene expression, employing ultrasensitive small-molecule drugs targeted toward nucleic acids. Gene expression can be regulated by
using chemicals to target transitional changes in the helical conformations of single-stranded (ss-) and double-stranded (ds-) DNA (e.g

4 comments | Blog It | Email It | Keywords: RNA, DNA,GENE single-stranded DNA gene expression microarrays RNA-DNA hybrid stability gene expression microarray Nuclear Gene Expression gene expression profile RNA, DNA,GENE Single-Stranded DNA gene expression microarrays RNA-DNA hybrid stability gene expression microarray Nuclear Gene Expression gene expression profile

Expression of the zebrafish intermediate neurofilament Nestin in the developing nervous system and in neural proliferation zones at postembryonic stages

Reviver submitted, created time 1 year 5 months (www.biomedcentral.com)

"This data demonstrate that in zebrafish, like in mammals, the expression of the intermediated neurofilament nestin gene may serve as a marker for stem cells and proliferating precursors in the developing embryonic nervous system as well as in the postembryonic brain."

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Robust regression for periodicity detection in non-uniformly sampled time-course gene expression dataBMC Bioinformatics

annatto submitted, created time 1 year 6 months (www.biomedcentral.com)

Since uneven sampling occurs often in biological measurements, the robust methods developed in this paper are expected to have many uses. The regression based formulation of the periodicity detection problem easily adapts to non-uniform sampling. Using robust regression helps to reject inconsistently behaving data points.

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Gene expression profiles in skeletal muscle after gene electrotransfer

annatto submitted, created time 1 year 6 months (www.biomedcentral.com)

"The small and transient changes found in the gene expression profiles are of great importance, as this demonstrates that DNA electrotransfer is safe with minor effects on the muscle host cells. These findings are essential for introducing the DNA electrotransfer to muscle for clinical use. Indeed the HV+LV pulse combination used has been optimised to ensure highly efficient and safe DNA electrotransfer."

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New data on robustness of gene expression signatures in leukemia: comparison of three distinct total RNA preparation procedures

annatto submitted, created time 1 year 6 months (www.biomedcentral.com)

"The signatures in the different subtypes of leukemias, regardless of the different extraction methods used, account for the biggest source of variation in the data. Lysis of mononuclear cells, followed by lysate homogenization and RNA extraction represents the optimum method for robust gene expression data and is thus recommended for obtaining robust classification results in microarray studies in acute leukemias."

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DEEP—A tool for differential expression effector prediction

bioman submitted, created time 1 year 6 months (nar.oxfordjournals.org)

High-throughput methods for measuring transcript abundance, like SAGE or microarrays, are widely used for determining differences in gene expression between different tissue types, dignities (normal/malignant) or time points. Further analysis of such data frequently aims at the identification of gene interaction networks that form the causal basis for the observed properties of the systems under examination

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A global view of Staphylococcus aureus whole genome expression upon internalization in human epithelial cells

Reviver submitted, created time 1 year 6 months (www.biomedcentral.com)

"Researchers have developed an in vitro model using human lung epithelial cells that shows intracellular bacterial persistence for up to 2 weeks. Using an original approach we successfully collected and amplified low amounts of bacterial RNA recovered from infected eukaryotic cells. Transcriptomic analysis using an oligoarray covering the whole S. aureus genome was performed at two post-internalization times and compared to gene expression of non-internalized bacteria. No signs of cellular death were observed after prolonged internalization of Staphylococcus aureus 6850 in epithelial cells

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MicroRNA miR-181a correlates with morphological sub-class of acute myeloid leukaemia and the expression of its target genes in global genome-wide analysis

athena submitted, created time 1 year 8 months (www.nature.com)

"MicroRNAs (miRNAs) are short single-stranded RNAs that have a potentially important role in gene regulation. Using a quantitative real-time polymerase chain reaction assay specific to the mature miRNA, the expression level of a selected group of haematopoietic tissue-specific miRNAs was measured across a set of 30 primary adult acute myeloid leukaemia (AML) with a normal karyotype. The expression levels of each miRNA were correlated with the genome-wide mRNA expression profiles in the same leukaemias

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