A novel view of the transcriptome revealed from gene trapping in mouse embryonic stem cells

captainclaw submitted, created time 1 year 7 months (www.genome.org)

"Embryonic stem (ES) cells are pluripotent cell lines with the capacity of self-renewal and the ability to differentiate into specific cell types. Researchers performed the first genome-wide analysis of the mouse ES cell transcriptome using ~250,000 gene trap sequence tags deposited in public databases. They unveiled >8000 novel transcripts, mostly non-coding, and >1000 novel alternative and often tissue-specific exons of known genes. Experimental verification of the expression of these genes and exons by RT-PCR yielded a 70% validation rate

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