p66 mediates anoikis through RhoA

angelfish submitted, created time 1 year 3 months (www.jcb.org)

"Researchers find that cells that lack p66Shc display poorly formed focal adhesions and escape anoikis. However, reexpression of p66Shc restores anoikis through a mechanism requiring focal adhesion targeting and RhoA activation but not an intact cytochrome c–binding motif. This pathway stimulates the formation of focal adhesions and stress fibers in attached cells and tension-dependent cell death upon detachment. p66Shc may thus report attachment status to the cell by imposing a tension test across candidate anchorage points, with load failure indicating detachment. "

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Cre Recombinase Gene Transfer In Vitro and Detection of loxP-Dependent Recombination

saury submitted, created time 1 year 6 months (www.cshprotocols.org)

"Altering the genome of intact cells and organisms by site-specific DNA recombination has become an important gene-transfer methodology. The inclusion of exogenous recombinase target sequences within transferred DNA segments allows subsequent modifications to previously altered genomic structure that increase the utility of gene transfer and enhance experimental design

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