The SNARE Complex from Yeast Is Partially Unstructured on the Membrane

jerry submitted, created time 4 months 2 weeks (www.structure.org)

Molecular recognition between cognate SNAREs leads to the formation of a four-helix bundle, which facilitates vesicle docking and membrane fusion. For a SNARE system involved in trafficking in yeast, target membrane (t-) SNARE Sso1p and vesicle associated (v-) SNARE Snc2p contribute one SNARE motif each, whereas another t-SNARE (Sec9) donates two N-terminal and C-terminal SNARE motifs (SN1 and SN2) to the helical bundle. By use of EPR, it is found that SN2 has a tendency to be uncoiled, leaving a significant population of the SNARE complexes to be partially unstructured on the membrane

2 comments | Blog It | Email It | Keywords: Sso1p Snc2p Sec9 membranes vesicle docking SNARE membrane fusion SN2 SNARE protein

Genome-Wide Annotation of Remorins, a Plant-Specific Protein Family: Evolutionary and Functional Perspectives

jane2007 submitted, created time 1 year 3 weeks (www.plantphysiol.org)

Remorins were discovered in a screen for plasma membrane (PM) proteins differentially phosphorylated in the presence of oligogalacturonides .The first remorin was initially designated as pp34, as it corresponded to a phosphorylated protein with a molecular mass of 34 kD in purified PM preparations from potato. After a section in J.L. Borge's "Book of Imaginary Beings," in which the remora fish is described to attach itself to larger fish and ships, this protein was renamed remorin due to its hydrophilic profile and its ability to attach to PMs.

1 comment | Blog It | Email It | Keywords: Remorins membranes