Direct glucocorticoid receptor–Stat5 interaction in hepatocytes controls body size and maturation-related gene expression

julie submitted, created time 1 year 7 months (www.genesdev.org)

"The glucocorticoid receptor regulates transcription through DNA binding as well as through cross-talk with other transcription factors. In hepatocytes, the glucocorticoid receptor is critical for normal postnatal growth. Using hepatocyte-specific and domain-selective mutations in the mouse we show that Stat5 in hepatocytes is essential for normal postnatal growth and that it mediates the growth-promoting effect of the glucocorticoid receptor through a direct interaction involving the N-terminal tetramerization domain of Stat5b

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Analyzing microarray data using CLANS

seanangel submitted, created time 1 year 7 months (bioinformatics.oxfordjournals.org)

Analysis of microarray experiments is complicated by the huge amount of data involved. Searching for groups of co-expressed genes is akin to searching for protein families in a database as, in both cases, small subsets of genes with similar features are to be found within vast quantities of data. CLANS was originally developed to find protein families in large sets of amino acid sequences where the amount of data involved made phylogenetic approaches overly cumbersome

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Hepatic gene expression changes in hypothyroid juvenile mice

broadcast submitted, created time 1 year 8 months (endo.endojournals.org)

"The molecular mechanisms involved in the response of developing mice to disruptions in maternal thyroid hormone (TH) homeostasis are poorly characterized. We used DNA microarrays to examine a broad spectrum of genes from the livers of mice rendered hypothyroid by treating pregnant mice from gestational day (GD) 13 to post-natal day (PND) 15 with 6-propyl-2-thiouracil (PTU) in drinking water."

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Gene expression shift towards normal B cells, decreased proliferative capacity and distinct surface receptors characterize leukemic blasts persisting during induction therapy in childhood acute lymphoblastic leukemia

athena submitted, created time 1 year 8 months (www.nature.com)

"The differentiation shift and low proliferative activity in d8 blasts may account for the persistence of blasts during therapy and affect their sensitivity to further therapeutic treatment. CD11b and CD119 are potential specific markers for d8 blast persistence and detection of minimal residual disease, which warrant further investigation."

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A meta-analysis of kidney microarray datasets

Scarlett submitted, created time 1 year 9 months (www.biomedcentral.com)

Microarrays provide a means to simultaneously examine the gene expression of the entire transcriptome in a single sample. Many studies have highlighted the need for novel software and statistical approaches to assess the measured gene expression. Less attention has been directed toward whether genes considered undetectable by microarray can be detected by other strategies or whether these genes offer accurate gene expression determinations. Recently, it was suggested that when analyzing microarray data from human kidney samples genes generally expressed at low abundance (i.e

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Patterns of gene expression in the limbic system of suicides with and without major depression

addict submitted, created time 1 year 9 months (www.nature.com)

The limbic system has consistently been associated with the control of emotions and with mood disorders. The goal of this study was to identify new molecular targets associated with suicide and with major depression using oligonucleotide microarrays in the limbic system (amygdala, hippocampus, anterior cingulate gryus (BA24) and posterior cingulate gyrus (BA29)). A total of 39 subjects were included in this study. They were all male subjects and comprised 26 suicides (depressed suicides=18, non depressed suicides=8) and 13 matched controls

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Genenext term capture and random amplification for quantitative recovery of homo

laoheqon submitted, created time 1 year 11 months (www.sciencedirect.com)

The polymerase chain reaction (PCR) is instrumental in molecular analysis of microorganisms, allowing for the selective amplification of nucleic acids directly from clinical and environmental samples. However, the principles that allow for targeted amplification of DNA become a hindrance when attempting to simultaneously discriminate and quantify complex mixtures of homologous previous termgenes.next term Here we present a simple solution to the quantitative problem by separating the enrichment and amplification aspects of a conventional PCR reaction

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