Stochastic inhibitor release and binding from single-enzyme molecules

herry submitted, created time 1 year 2 months (www.pnas.org)

Inhibition kinetics of single-β-galactosidase molecules with the slow-binding inhibitor D-galactal have been characterized by segregating individual enzyme molecules in an array of 50,000 ultra small reaction containers and observing substrate turnover changes with fluorescence microscopy.

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