Using ChIP-chip technology to reveal common principles of transcriptional repression in normal and cancer cells

sea-maid submitted, created time 8 months 1 week (www.genome.org)

In this paper, the authors performed chromatin immunoprecipitation using antibodies to H3me3K27, H3me3K9, 5-methyl-cytosine, and POLR2A. And then they found that (1) the percentage of low expressed genes bound by POLR2A, H3me3K27, H3me3K9, or 5-methyl-cytosine is similar in all 12 cell types, regardless of differentiation or neoplastic state; (2) a gene is generally repressed by only one mechanism; and (3) distinct classes of genes are repressed by certain mechanisms

1 comment | Blog It | Email It | Keywords: gene expression chromatin silening gene chip transcriptional repression