315 Articles with the topic: Methodology of Basic Research
Fusing Embryonic Stem Cells with Adult Cells Using Highly Efficient New Fusing System
piggy submitted, created time 3 days 21 hours (www.sciencedaily.com)
MIT engineers have developed a new, highly efficient way to pair up cells so they can be fused together into a hybrid cell.
The new technique should make it much easier for scientists to study what happens when two cells are combined. For example, fusing an adult cell and an embryonic stem cell allows researchers to study the genetic reprogramming that occurs in such hybrids 
Drilling Holes Through Deadly Bacteria's Kevlar-like Hide
piggy submitted, created time 1 week 4 days (www.sciencedaily.com)
To protect themselves from human defenses, disease-causing bacteria have evolved a cell wall made from a nearly impenetrable tangle of tightly woven strands. That’s made it difficult for scientists to see what goes on inside these potentially deadly organisms. But that era is now over. Rockefeller University researchers have now figured out how to drill holes through the Kevlar-like hide of gram-positive bacteria without obliterating them, and in doing so, they’ve made it possible to study, from the inside out, most of the known bacteria on the planet.
The work, led by Vincent A
Recipe for capturing authentic embryonic stem cells may apply to any mammal, study suggests
piggy submitted, created time 1 week 6 days (www.eurekalert.org)
Researchers have what they think may be a basic recipe for capturing and maintaining indefinitely the most fundamental of embryonic stem cells from essentially any mammal, including cows, pigs and even humans. Two new studies reported in the December 26th issue of the journal Cell, a Cell Press publication, show that a cocktail first demonstrated to work in mice earlier this year, which includes inhibitory chemicals, also can be used to successfully isolate embryonic stem cells from rats.
Authentic rat embryonic stem cells had never before been established
New embryonic stem cells ratted out
sea-maid submitted, created time 2 weeks 7 hours (www.sciencenews.org)
Scientists have finally succeeded in deriving embryonic stem cells from rats, providing the research community with a new tool for modeling human disease. The method used may prove to be a general recipe to create stem cells from many different animals, the researchers say. The findings appear in two companion papers in the Dec. 26 Cell.
New technique is quantum leap forward in understanding proteins
piggy submitted, created time 2 weeks 1 day (www.eurekalert.org)
In this ongoing quest, a group of Scripps Research Institute scientists, along with colleagues from the University of California, San Diego, (UCSD) have borrowed from physics to deliver one of those research rarities—an unmitigated success. The group has devised a computational method that, with remarkable accuracy, predicts how bacterial proteins fold and interact
Researchers advance knowledge of little nano-machines in our bodies
piggy submitted, created time 2 weeks 6 days (www.eurekalert.org)
A discovery by Canada-U.S. biophysicists will improve the understanding of ion channels, akin to little "nano-machines" or "nano-valves" in our body, which when they malfunction can cause genetic illnesses that attack muscles, the central nervous system and the heart.
As reported in the current issue of the Proceedings of the National Academy of Sciences (PNAS), researchers from the Université de Montréal and the University of Chicago have developed a novel method to detect the movement of single proteins that control the ion exchange between the cells and their environment
Data organization and the modern laboratory
Darkfrog submitted, created time 3 weeks 1 day (judson.blogs.nytimes.com)
This is a New York Times blog post with lots of advice about how to organize data for presentation. Specifically, it's a review of the computer programs Zotero (which is basically Pandora but for scientific papers) and Papers.
Frankly, I don't see the big deal. The writer is talking about how organization got much harder after the journals all went digital. This isn't a new problem. When I was in elementary school, my teachers told me, "When I was a kid, the problem was finding the information. Yours is organizing it
Nanotubes Sniff Out Cancer Agents in Living Cells
piggy submitted, created time 3 weeks 2 days (www.sciencedaily.com)
MIT engineers have developed carbon nanotubes into sensors for cancer drugs and other DNA-damaging agents inside living cells.
The sensors, made of carbon nanotubes wrapped in DNA, can detect chemotherapy drugs such as cisplatin as well as environmental toxins and free radicals that damage DNA.
"We've made a sensor that can be placed in living cells, healthy or malignant, and actually detect several different classes of molecules that damage DNA," said Michael Strano, associate professor of chemical engineering and senior author of a paper on the work appearing in the Dec
Researchers Develop a Technique for Counting Messages Made by Single Genes
piggy submitted, created time 3 weeks 6 days (www.sciencedaily.com)
Researchers from Albert Einstein College of Medicine of Yeshiva University have described a technique for looking more precisely at a fundamental step of a cell's life — a gene, DNA, being read into a message, mRNA. The technique could provide a window into the process by which genes are switched on inappropriately, causing disease.
The new technique provides a detailed look into processes that until now were proven but never visualized
Inner Workings of the Immune System Filmed
piggy submitted, created time 1 month 3 days (www.sciencedaily.com)
Forget what's number one at the box office this week. The most exciting new film features the intricate workings of the body, filmed by scientists using ground-breaking technology.
For the first time in Australia, scientists at Sydney's Centenary Institute have filmed an immune cell becoming infected by a parasite and followed the infection as it begins to spread throughout the body
HIV testing on the cheap! ...but no one's interested.
Darkfrog submitted, created time 1 month 3 days (www.nature.com)
The standard HIV test, such as a patient might receive in a hospital in New York or Paris, is generally too expensive for AIDS prevention organizations in Africa. However, new developments that could make HIV testing more feasible are not garnering enough interest from private investors.
These tests are not limited to determining who is infected and who isn't. For example, CD4 tests, which measure HIV-indicative changes in the patient's cell receptors, are used to tell how far the disease has progressed, which can help doctors give their patients the most effective drug regimens
A Brain Circuit for Bungee Jumping?
sea-maid submitted, created time 1 month 1 week (sciencenow.sciencemag.org)
Scientists have known for some time that the white matter in our brains--the strands of nerve fibers that connect nerve cell bodies, or gray matter--serve as the wires through which neural information flows. However, figuring out exactly which parts of the brain connect to each other, and how strong these connections are, has only been possible recently in living humans thanks to a technique called diffusion tensor imaging. A type of magnetic resonance imaging, the method traces the web of white matter by following the diffusion of fluid through the nerve fibers
Microcapsules Act As "Roach Motel" to Harmful Bacteria
piggy submitted, created time 1 month 2 weeks (www.sciencedaily.com)
Scientists at the University of Florida and the University of New Mexico have created tiny microscopic spheres that trap and kill harmful bacteria in a manner the scientists liken to “roach motels” snaring and killing cockroaches. The research could lead to new coatings that will disinfect common surfaces, combat bioterrorism or sterilize medical devices, reducing the devices’ responsibility for an estimated 1.4 million infection-related deaths each year
Well that's one way to present your dissertation...
Darkfrog submitted, created time 1 month 2 weeks (sciencenow.sciencemag.org)
This isn't quite as serious as our usual DiscoveR8 fare, but it has potential implications for science education and public image. You guys have often heard me give Science magazine the dubious praise, "You would NEVER see this in Nature." Something else that I say all the time, though, is, "My feelings can only be expressed through dance."
Science magazine sent out a rather interesting call for not-exactly-papers. The challenge was "Tell us about your Ph.D. research ...through dance
Are plastic tools spoiling experimental results?
sea-maid submitted, created time 1 month 4 weeks (www.nature.com)
Thousands of scientists could be unwittingly ruining their own experiments merely by using standard plastic lab equipment, according to a new study. These findings may have strong implications for the methodology of basic research.
Andrew Holt, a researcher at the University of Alberta in Edmonton, Canada, was looking at how drugs affected the human enzyme monoamine oxidase B when he noticed that the drugs seemed to be inhibiting enzyme activity at much lower concentrations than they should
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